I. 原著論文

39. K.Yuyama, S. Mitsutake, and Y. Igarashi.
Pathological roles of ceramide and its metabolites in metabolic syndrome and Alzheimer's disease
BBA - Molecular and Cell Biology of Lipids Online publication complete (2013)

38. I. Murakami, S. Mitsutake, N. Kobayashi, J. Matsuda, A. Suzuki, T. Shigyo, and Y. Igarashi
Oral administration of phytosphingosine improves high-fat diet-induced glucose intolerance
Bioscience, Biotechnology, and Biochemistry (2012)in press

37. M. Taniguchi, K. Kitatani, T. Kondo, M.H. Nishimura, S. Asano, A. Hayashi, S. Mitsutake, Y. Igarashi, H. Umehara, T. Hiroyuki, J. Kigawa, and T. Okazaki
Regulation of autophagy and its associated cell death by sphingolipid rheostat: reciprocal role of ceramide and sphingosine-1-phosphate in the mTOR pathway
Journal of Biological Chemistry (2012) in press

36. K. Zama, S. Mitsutake, K. Watanabe, T. Okazaki, and Y. Igarashi
A sensitive cell-based method to screen for selective inhibitors of SMS1 or SMS2 using HPLC and a fluorescent substrate
Chemistry and Physics of Lipids (2012) in press

35. Y. Shirakura, K. Kikuchi, K. Matsumura, K. Mukai, S. Mitsutake, and Y. Igarashi
4, 8-Sphingadienine and 4-hydroxy-8-sphingenine activate ceramide production in the skin
Lipids in Health and Disease (2012) 11, 108

34. S. Asano, K. Kitatani, M. Taniguchi, M. Hashimoto, K. Zama, S. Mitsutake, Y. Igarashi, M. Takeya, A. Hayashi, H. Umehara, and T. Okazaki
Regulation of cell migration by sphingomyelin synthase: Sphingomyelin in lipid rafts decreases responsiveness to signaling by the CXCL12/CXCR4 pathway
Molecular and Cellular Biology (2012)32, 3242-3252

33. S. Mitsutake, T. Date, H. Yokota, M. Sugiura, T. Kohama, and Y. Igarashi
Ceramide kinase deficiency improve diet-induced obesity and insulin resistance
FEBS Letters (2012) 586 ,1300-1305.

32. K. Yuyama, H. Sun, S. Mitsutake, and Y. Igarashi
Sphingolipid-modulated exosome secretion promotes the clearance of amyloid-β by microglia
Journal of Biological Chemistry (2012) 287 ,10977-10989.

31. S. Mitsutake, K. Zama, H. Yokota, T. Yoshida, M. Tanaka, M. Mitsui, M. Ikawa, M. Okabe, Y. Tanaka, T. Yamashita, H. Takemoto, T. Okazaki, K. Watanabe, and Y. Igarashi
Dynamic modification of sphingomyelin in lipid microdomains controls development of obesity, fatty liver, and type 2 diabetes
Journal of Biological Chemistry (2011)286 ,28544-28555.

30. N. Fujitani, Y. Takegawa, K. Araki, J. Furukawa, S. Mitsutake, Y. Igarashi, M. Ito, and Y. Shinohara
Qualitative and Quantitative Cellular Glycomics of Glycosphingolipids Based on Rhodococcal EGCase I and II-Assisted Glycan Cleavage, Glycoblotting-Assisted Sample Preparation and MALDI-TOF /TOF MS Analysis
Journal of Biological Chemistry (2011)286 ,41669-41679.

29. I. Murakami, Y. Wakasa, S. Yamashita, T. Kurihara, K. Zama, N. Kobayashi, Y. Mizutani, S. Mitsutake, T. Shigyo , and Y. Igarashi
Phytoceramide and sphingoid bases derived from brewer's yeast Saccharomyces pastorianus activate peroxisome proliferator-activated receptors
Lipids in Health and Disease(2011)10 ,150

28. Y. Ohno, S. Suto, M. Yamanakaa, Y. Mizutani, S. Mitsutake, Y. Igarashi Y, T. Sassa, and A. Kihara
ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis
Proceedings of the National Academy of Sciences of the United States of America(2010)107 ,18439-18444.

27. S. Mitsutake, C. Suzuki, M. Akiyama, K. Tsuji, T. Yanagi, H. Shimizu, and Y. Igarashi
ABCA12 dysfunction causes a disorder in glucosylceramide accumulation during keratinocyte differentiation
Journal of Dermatological Science(2010) 60 ,128-129.

26. S. Mitsutake, H. Kumada, M. Soga, Y. Hurue, F. Asanuma, M. Nagira, M. Deguchi, T. Date, U. Yokose, Y. Inagaki, M. Sugiura, T. Kohama, and Y. Igarashi
Ceramide kinase is not essential but might act as an Ca(2+)-sensor for mast cell activation
Prostaglandins and Other Lipid Mediators(2010)93 ,109-112.

25. K. Tsuji, S. Satoh, S. Mitsutake, I. Murakami, JJ. Park, Q. Li, YT. Chang, SK. Chung, and Y. Igarashi
Evaluation of synthetic sphingolipid analogs as ligands for peroxisome proliferator-activated receptors
Bioorganic and Medicinal Chemistry Letters(2009)19 ,1643-1646.

24. K. Tsuji, S. Mitsutake, U. Yokose, M. Sugiura, T. Kohama, and Y. Igarashi
Role of ceramide kinase in peroxisome proliferator-activated receptor beta-induced cell survival of mouse keratinocytes
FEBS Journal (2008)275, 3815-3826.

23. T. Tomiyama, S. kaihou, M. Ishida , H. Nishikawa, N. Yamazaki, K. Tsuji, S. Mitsutake, and Y. Igarashi
The water retention effects and action for atopic dermatitis-like symptoms of ethyl alcohol extracts ( from Tamogi-take mushroom) on animal model of atopic dermatitis.

22. S. Mitsutake, U. Yokose, M. Kato, I. Matsuoka, J. M. Yoo, T. J. Kim, H. S. Yoo, K. Fujimoto, Y. Ando, M. Sugiura, T. Kohama, and Y. Igarashi
The generation and behavioral analysis of ceramide kinase-null mice, indicating a function in cerebellar Purkinje cells
Biochemical and Biophysical Research Communications(2007)363, 519-524.

21. S. Mitsutake, and Y. Igarashi
Transbilayer movement of ceramide in the plasma membrane of live cells
Biochemical and Biophysical Research Communications(2007)359, 622-627.

20. H. Kumada, S. Mitsutake, Y. Inagaki, S. Mitsunaga, H. Tsuchikawa, S. Katsumura, and Y. Igarashi
Kinetics of the ceramide kinase inhibitor K1, a suppressor of mast-cell activation
Bioscience, Biotechnology, and Biochemistry(2007)71, 2581-2584

19. T. Date, S. Mitsutake, and Y. Igarashi
Ceramide kinase expression is altered during macrophage-like cell differentiation of the leukemia cell line HL-60
In Vitro Cellular and Developmental Biology-Animal (2007)43, 321-323

18. K. Tsuji, S. Mitsutake, J. Ishikawa, Y. Takagi, M. Akiyama, H. Shimizu, T. Tomiyama, and Y. Igarashi
Dietary glucosylceramide improves skin barrier function in hairless mice
Journal of Dermatological Science(2006)44, 101-107.

17. S. Takeda, S. Mitsutake, K. Tsuji, and Y. Igarashi
Apoptosis occurs via the ceramide recycling pathway in human HaCaT keratinocytes
Journal of Biochemistry(2006)139, 255-262.

16. T. J. Kim, S. Mitsutake, and Y. Igarashi
The interaction between the pleckstrin homology domain of ceramide kinase and phosphatidylinositol 4,5-bisphosphate regulates the plasma membrane targeting and ceramide 1-phosphate levels
Biochemical and Biophysical Research Communications(2006)342, 611-617.

15. Y. Inagaki, S. Mitsutake, and Y. Igarashi
Identification of a nuclear localization signal in the retinitis pigmentosa-mutated RP26 protein, ceramide kinase-like protein
Biochemical and Biophysical Research Communications(2006)343, 982-987.

14. S. Mitsutake, and Y. Igarashi
Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor
Journal of Biological Chemistry(2005)280, 40436-40441.

13. T. J. Kim, S. Mitsutake, M. Kato, and Y. Igarashi
The leucine 10 residue in the pleckstrin homology domain of ceramide kinase is crucial for its catalytic activity
FEBS Letters(2005)579, 4383-4388.

12. J. W. Kim, Y. W. Kim, Y. Inagaki, Y. A. Hwang, S. Mitsutake, Y. W. Ryu, W. K. Lee, H. J. Ha, C. S. Park, and Y. Igarashi
Synthesis and evaluation of sphingoid analogs as inhibitors of sphingosine kinases
Bioorganic and Medicinal Chemistry(2005)13, 3475-3485.

11. J. W. Kim, Y. Inagaki, S. Mitsutake, N. Maezawa, S. Katsumura, Y. W. Ryu, C. S. Park, M. Taniguchi, and Y. Igarashi
Suppression of mast cell degranulation by a novel ceramide kinase inhibitor, the F-12509A olefin isomer K1
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids(2005)1738, 82-90.

10. S. Yokota, Y. Taniguchi, A. Kihara, S. Mitsutake, and Y. Igarashi
Asp177 in C4 domain of mouse sphingosine kinase 1a is important for the sphingosine recognition
FEBS Letters (2004)578, 106-110.

9. S. Mitsutake, T. J. Kim, Y. Inagaki, M. Kato, T. Yamashita, and Y. Igarashi
Ceramide kinase is a mediator of calcium-dependent degranulation in mast cells
Journal of Biological Chemistry(2004)279, 17570-17577.

8. Y. Inagaki, P. Y. Li, A. Wada, S. Mitsutake, and Y. Igarashi
Identification of functional nuclear export sequences in human sphingosine kinase 1
Biochemical and Biophysical Research Communications(2003)311, 168-173.

7. M. Furusato, N. Sueyoshi, S. Mitsutake, K. Sakaguchi, K. Kita, N. Okino, S. Ichinose, A. Omori, and M. Ito
Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8
Journal of Biological Chemistry(2002)277, 17300-17307.

6. E. Romiti, E. Meacci, G. Tanzi, L. Becciolini, S. Mitsutake, M. Farnararo, M. Ito, and P. Bruni
Localization of neutral ceramidase in caveolin-enriched light membranes of murine endothelial cells
FEBS Letters(2001)506, 163-168.

5. S. Mitsutake, M. Tani, N. Okino, K. Mori, S. Ichinose, A. Omori, H. Iida, T. Nakamura, and M. Ito
Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney
Journal of Biological Chemistry (2001)276, 26249-26259.

4. M. Tani, N. Okino, S. Mitsutake, T. Tanigawa, H. Izu, and M. Ito
Purification and
characterization of a neutral ceramidase from mouse liver. A single protein catalyzes the reversible reaction in which ceramide is both hydrolyzed and synthesized
Journal of Biological Chemistry(2000)275, 3462-3468.

3. M. Tani, N. Okino, S. Mitsutake, and M. Ito
Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide
Journal of Biochemistry(1999)125, 746-749.

2. S. Mitsutake, K. Kita, T. Nakagawa, and M. Ito
Enzymatic synthesis of [14C] glycosphingolipids by reverse hydrolysis reaction of sphingolipid ceramide N-deacylase: detection of endoglycoceramidase activity in a seaflower
Journal of Biochemistry (1998)123, 859-863.

1. S. Mitsutake, K. Kita, N. Okino, and M. Ito
[14C] ceramide synthesis by sphingolipid ceramide N-deacylase: new assay for ceramidase activity detection
Analitical Biochemistry(1997)247, 52-57.

II. 著書/総説

14. 光武進、村上逸雄、五十嵐靖之
アレルギーの臨床(2013) 33, 165-169.

13. S. Mitsutake, Y. Igarashi
Sphingolipids in lipid microdomains and obesity
Vitamins & Hormones(2013) 91, 1. "Obesity" Edited by Gerald Litwack

12. 村上逸雄、光武進、小林直之、執行達朗、五十嵐靖之
アレルギーの臨床(2012) 32, 77-81.

11. 光武進、五十嵐靖之
バイオサイエンスとインダストリー(2012) 70, 102-107.

10. 光武進、五十嵐靖之
「セラミド-基礎と応用-」ここまできたセラミド研究最前線(2011) 186-191.

9. Y. Mizutani,S. Mitsutake, K. Tsuji, A. Kihara, Y. Igarashi.
Ceramide biosynthesis in keratinocyte and its role in skin function.
Biochimie(2009)91, 784-790.

8. 沖野望、谷元洋、光武進、吉村征活、合田初美、伊東信
細胞(2009)41, 174-177.

7. 水谷有紀子、光武進、五十嵐靖之
細胞(2009)41, 182-185.

6. A. Kihara,S. Mitsutake, Y. Mizutani, Y. Igarashi.
Metabolism and biological functions of two phosphorylated sphingolipids, sphingosine 1-phosphate and ceramide 1-phosphate.
Progress in Lipid Research(2007)46, 126-144.

5. S. Mitsutake, T. J. Kim, Y. Igarashi.
Ceramide 1-phosphate
in Sphingolipid Biology(2006)209-215, Springer(Tokyo)
Edited by Hirabayashi Y., Igarashi Y., Merrill A.H.

4. 光武進、五十嵐靖之
バイオサイエンスとインダストリー(2006)64, 36-37

3. M. Ito, N. Okino, M. Tani, S. Mitsutake, K. Kita.
Molecular Evolution of Neutral Ceramidase: From Bacteria to Mammals
in Ceramide Signaling(2003)41-48, Landes Bioscience
Edited by Futerman A.H.

2. 伊東信、沖野望、谷元洋、光武進、森薫
中性セラミダーゼの分子進化 - 病原因子とシグナリング分子 -
蛋白質核酸酵素(2002)47, 455-462.

1. M. Ito, S. Mitsutake, M. Tani, K. Kita.
Enzymatic synthesis of [14C]ceramide, [14C]glycosphingolipids, and omega-aminoceramide.
Methods in Enzymology(2000)311, 682-689.

III. その他の論文・解説等

3. 光武進、五十嵐 靖之
化学(化学同人発行)(2007)62, 72

2. T. J. Kim, S. Mitsutake, Y. Igarashi.
Ceramide 1-phosphate.
BioWave 化学(Korean review)(2005)7, 1-11.

1. 光武進
ファルマシア(日本薬学会発行)(2003)39, 162-163

IV. 特許

8. 〈出願中につき詳細未公開〉

7. 〈出願中につき詳細未公開〉

6. スフィンゴミエリン合成酵素SMS2の生理機能を応用したメタボリックシンドローム改善、又は改善薬のスクリーニング方法

6-(a). Amelioration of metabolic syndrome or method for screening for ameliorating agent for metabolic syndrome utilizing physiological function of sphingomyelin synthase SMS2
S. Mitsutake, Y. Igarashi, K. Zama, T. Yoshida, Y. Tanaka, H. Takemoto
出願人:National University Corporation Hokkaido University and Shionogi & Co., Ltd

5. 新規PPARsアゴニスト

4. セラミドキナーゼ阻害化合物およびそれを有効成分とする医薬組成物

3. セラミドキナーゼ阻害剤およびその利用

2. Sphingolipid ceramide N-deacylase, method for production sphingolipids and sphingolipid derivatives, and sphingolipid ceramide N-deacylase gene
M. Ito, T. Kurita, K. Kita, N. Sueyoshi, S. Mitsutake, M. Fujita, N. Okino, H. Izu, I. Kato
出願人:Takara Bio. Inc.

1. スフィンゴ脂質及びスフィンゴ脂質誘導体の製造法

1-(a). Process for the preparation of sphingolipids and sphingolipid derivatives
M. Ito, T. Kurita, S. Mitsutake, K. Kita
出願人:Takara Shuzo Co., Ltd.